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Electron Microscopy of Astasia longa *
Author(s) -
RINGO DAVID L.
Publication year - 1963
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1963.tb01657.x
Subject(s) - uranyl acetate , euglena gracilis , endoplasmic reticulum , golgi apparatus , nucleolus , electron microscope , nuclear membrane , negative stain , biophysics , mitochondrion , membrane , ribosome , chemistry , nucleus , biology , microbiology and biotechnology , ultrastructure , anatomy , chloroplast , biochemistry , physics , optics , rna , gene
SYNOPSIS. Cells of Astasia longa (Jahn) were grown on a simple defined medium, fixed in the stationary phase of growth with OsO 4 , stained with uranyl acetate, and embedded in Vestopal for electron microscopy. The cell wall was found to be a membranous structure 100 Å thick. A double nuclear membrane, nucleolus, and many darkly staining bodies were observed in the nucleus. Cells contained ribosomes and elaborate Golgi bodies, but no well developed endoplasmic reticulum. The numerous mitochondria typically had well‐defined cristae running longitudinally in the mitochondrion. Two bodies of unknown function were observed, one sometimes seen to surround mitochondria, the other bounded by a double membrane. A model of the reservoir structure of the euglenoids is presented, based on observations of the reservoir of A. longa and on published data from Euglena gracilis and E. viridis.