Premium
Comparative in vitro evaluation of apheresis platelets stored with 100% plasma versus bicarbonated Ringer's solution with less than 5% plasma
Author(s) -
Oikawa Shinji,
Sasaki Dai,
Kikuchi Masaki,
Sawamura Yoshihiro,
Itoh Takashi
Publication year - 2013
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2012.03773.x
Subject(s) - chemistry , bicarbonate , citric acid , platelet , sodium bicarbonate , sodium , trisodium citrate , chromatography , magnesium , apheresis , nuclear chemistry , biochemistry , medicine , organic chemistry
BACKGROUND: The major strategy for reducing the frequency of adverse reactions to platelet (PLT) transfusions is PLT washing with PLT additive solutions (PASs). In Japan, a mixture of medical infusion solutions such as acetate Ringer's solution, sodium bicarbonate, magnesium sulfate, and ACD‐A is currently used as a PAS because none of the common types of PASs are officially permitted for clinical use. Recently, a bicarbonated Ringer's solution (BRS) was developed using bicarbonate as an alkaline agent. The aim of this study was to evaluate whether a BRS can effectively be utilized as a PAS for clinical use. STUDY DESIGN AND METHODS: The washing and storage solution was prepared by adding 25 mL ACD‐A to 500 mL of BRS (BRS‐A), consisting of 95.2 mmol/L NaCl, 3.8 mmol/L KCl, 0.9 mmol/L MgCl 2 ,1.4 mmol/L CaCl 2 , 26.6 mmol/L NaHCO 3 , 5.8 mmol/L glucose, 4.2 mmol/L trisodium citrate, and 1.8 mmol/L citric acid. The in vitro properties of apheresis PLTs suspended in BRS‐A with low concentration of plasma (<5%) were compared with those suspended in 100% plasma during 7‐day storage. RESULTS: The in vitro properties of pH, hypotonic shock response, glucose consumption rate, lactate production rate, swirling, CD62P, and CD42b expression in PLTs suspended in BRS‐A were comparable or superior to those suspended in 100% plasma during 7‐day storage. CONCLUSION: BRS‐A, prepared by mixing the only two solutions permitted for clinical use in Japan, has a positive capability to maintain PLT function. These results indicate that PLT washing and storage with BRS‐A is feasible.