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Use of polyethylene glycol for performing autologous adsorptions
Author(s) -
Cid Joan,
Ortín Xavier,
Pinacho Asunción,
Parra Rafael,
Contreras Enric,
Elies Enric
Publication year - 2005
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2005.04369.x
Subject(s) - peg ratio , polyethylene glycol , adsorption , autoantibody , chemistry , chromatography , antibody , medicine , immunology , biochemistry , organic chemistry , finance , economics
BACKGROUND: Our reference adsorption procedure is to autoadsorb serum with ZZAP‐pretreated patients’ red cells (RBCs), although it is time‐consuming. The aim of this study was to evaluate the use of polyethylene glycol (PEG) for performing autologous adsorption procedures without pretreatment of patients’ RBCs. STUDY DESIGN AND METHODS: Equal volumes of patient's plasma, patient's RBCs, and PEG were mixed. This mixture was incubated for 15 minutes at 37°C, and the adsorbed plasma‐PEG mixture was immediately harvested to proceed to the antiglobulin test with anti‐immunoglobulin G. The ZZAP‐adsorption procedure was performed in our reference laboratory. RESULTS: Thirty‐one samples were detected with warm autoantibodies in pretransfusion testing. A total number of 58 autologous adsorption procedures were performed with PEG in 870 minutes (mean, 28 min) and alloantibodies were detected in 4 (13%) cases (anti‐E in 2 cases and anti‐E + K in 2 cases). Our reference laboratory performed a total number of 61 adsorption procedures with ZZAP in 3660 minutes (mean, 118 min) and detected the same alloantibodies specificities. CONCLUSION: The PEG autologous adsorption procedure is an efficient method of enhancing autoantibody adsorption and alloantibody detection and decreasing the labor‐intensive testing required by the presence of serum warm autoantibodies in pretransfusion samples.