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Immunologic Characterization of Anti‐H Isohemagglutinins
Author(s) -
Chattoraj A.,
Gilbert R.,
Josephson A. M.
Publication year - 1968
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1968.tb02438.x
Subject(s) - ouchterlony double immunodiffusion , sephadex , chromatography , immunodiffusion , iodoacetamide , chemistry , size exclusion chromatography , radial immunodiffusion , antibody , medicine , immunology , biochemistry , enzyme , cysteine , antiserum
The anti‐H agglutinins from three different O h persons (Bombay) have been examined. The methods included gel filtration on Sephadex G‐200, DEAE‐Sephadex anion exchange chromatography, preparation of saline eluates, Ouchterlony method of immunodiffusion, and treatment with 2‐mercaptoethanol‐iodoacetamide. The anti‐H agglutinins were found to be associated with 19S IgM.