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Diethyl Ether Inhibits Ethanol Metabolism in Vivo by Interaction with Alcohol Dehydrogenase
Author(s) -
Normann Per Trygve,
Ripel Åse,
Mørland Jørg
Publication year - 1987
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1987.tb01282.x
Subject(s) - alcohol dehydrogenase , ethanol , in vivo , alcohol , diethyl ether , chemistry , metabolism , ether , ethanol metabolism , biochemistry , alcohol oxidoreductase , enzyme , organic chemistry , nad+ kinase , biology , microbiology and biotechnology
The ethanol disappearance rate was determined in fed rats given 20‐40 mM ethanol and anesthetized with pentobarbital (control group) and diethyl ether. The control ethanol disappearance rate was 0.27 ± 0.02 mM/min (±SD, n = 4). Rats anesthetized with diethyl ether (blood levels of 9–13 mM) revealed an ethanol disappearance rate of 0.13 ± 0.05 mM/min (±SD, n = 7), i.e., 52% inhibition of the control rate. Kinetic studies on crystalline and lyophilized alcohol dehydrogenase from equine liver demonstrated that 20 mM diethyl ether inhibited the initial rate of ethanol oxidation by 55%. By using ethanol as the variable substrate the inhibition of alcohol dehydrogenase was described by a mixed noncompetitive/uncompetitive mechanism.