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Evidence for a Direct Inhibitory Effect of Ethanol upon Gonadotropin Secretion at the Pituitary Level
Author(s) -
Schade Robert R.,
Bonner Gregory,
Gay Ver C.,
Thiel David H. Van
Publication year - 1983
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1983.tb05430.x
Subject(s) - medicine , trypan blue , endocrinology , secretion , incubation , ethanol , basal (medicine) , in vitro , pituitary gland , hormone , gonadotropin , chemistry , pituitary hormones , biology , biochemistry , insulin
Mixed rat pituitary cells harvested from pituitaries obtained from adult male rats were maintained in tissue culture for periods up to 240 hr. Viability determined by trypan blue exclusion at the end of each experiment was greater than 90%. Basal secretory rats of luteiniring hormone (LH) into the culture medium were determined before and after the addition of ethanol (300 mg/100 ml) to the Incubation medium. Ethanol‐reduced (p <.01) LH Secretion of treated cultures were compared to control cultures. Based upon this preliminary in vitro study with rat tissue and on prior studies performed in man and animals, we suggest that ethanol inhibits LH secretion at the pituitary level.