Premium
Serum and Intracellular Detection of Cytokines in Patients Undergoing Chronic Hemodialysis
Author(s) -
Roccatello Dario,
Formica Marco,
Cavalli Guido,
Quattrocchio Giacomo,
Aimo Giuseppe,
Polloni Renato,
Amprimo Maria C.,
Moho Andrea,
Martha Guido,
Isidoro Ciro,
Mazengo Maurizia,
Coppo Rosanna,
Sena Luigi M.,
Piccoli Giuseppe
Publication year - 1992
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1992.tb00283.x
Subject(s) - hemodialysis , dialysis , tumor necrosis factor alpha , medicine , basal (medicine) , intracellular , cytokine , receptor , dialysis tubing , endocrinology , gastroenterology , immunology , membrane , chemistry , biochemistry , insulin
Abstract: Tumor necrosis factor (TNF) was detected, before and after dialysis, in sera from 69 patients and, at various times during dialysis, in 28 patients carefully selected for the absence of intercurrent illness. Blood samples were also sequentially collected for separation of monocytes, and cells were sonicated to detect intracellular TNF. Compared with serum levels obtained from 41 healthy subjects, basal TNF values of the unselected group of 69 patients were significantly higher (p < 0.01), independent of the dialyzer membrane. A significant increase in TNF levels by the end of dialysis was found only with Cuprophan (p < 0.01). In the selected group of 28 patients, no significant changes in TNF values were observed in sequential samples. However, a significant increase of intramonocyte TNF levels was found in Cuprophan patients (p < 0.025). Soluble interleukin‐2 receptor (IL‐2R) levels, measured in parallel in sera from unselected and selected patients, were found to be very much higher than healthy controls without significant changes during the dialysis procedure. While the diverse profiles of TNF obtained from differently selected patients suggest that mechanisms other than membrane biocompatibility play a role in the appearance of these low cytokine levels, the possible nature of uremic toxin for soluble IL‐2R can be envisaged by detection in dialysis patients.