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Vacuolating Cytotoxin A is Associated with Increased Thrombin Generation in Gastric Mucosa
Author(s) -
Suzuki Yuko,
Gabazza Esteban Cesar,
Imoto Ichiro,
James Roger F.L.,
Hirayama Toshiya,
Wada Akihiro,
Horiki Noriyuki,
Nakamura Misaki,
Inoue Hiroyuki,
Kuroda Mikito,
Ogura Ayumi,
Taguchi Yukiko,
Yano Yutaka,
Taguchi Osamu,
Suzuki Koji,
Adachi Yukihiko
Publication year - 2005
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1111/j.1523-5378.2005.00323.x
Subject(s) - thrombin , helicobacter pylori , gastric mucosa , in vivo , epidermal growth factor , coagulation , tissue factor , in vitro , medicine , chemistry , immunology , biology , stomach , receptor , platelet , biochemistry , microbiology and biotechnology
Background. Activation of the coagulation system is a critical response for both the repair of tissue injury and the host defense against microbial pathogens. Activation of the coagulation cascade culminates with the generation of thrombin. In vitro studies have shown that thrombin protects gastric epithelial cells from injury. The present study was undertaken to assess in vivo the relationship between gastric intramucosal generation of thrombin and Helicobacter pylori infection. Materials and methods. This study comprised 59 patients with gastroduodenal disorders. There were 27 patients with H. pylori infection ( Hp +), 14 without it ( Hp –), and 18 patients with cured H. pylori infection ( Hp c). The gastric intramucosal concentrations of thrombin–antithrombin complex (TAT), epidermal growth factor (EGF), prostaglandin E 2 (PGE 2 ), and vacuolating cytotoxin A (VacA) were measured by specific immunoassays. Results. The level of TAT was significantly increased in patients with Hp + compared to Hp – and Hp c. The levels of TAT, EGF and PGE 2 were higher in VacA (+) patients than in those with VacA (–). VacA induced significant expression of tissue factor in gastric epithelial cells in vitro. The gastric intramucosal level of VacA antigen was proportionally and significantly correlated with TAT, EGF and PGE 2 in Hp + patients. The level of TAT was proportionally and significantly correlated with EGF in Hp + patients but not in Hp – and HP c patients. Conclusions. These results showed that VacA produced by H. pylori is associated with increased thrombin generation, and that thrombin may play a protective role in H. pylori ‐associated gastroduodenal disorders.