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The effect of adrenalectomy on interleukin‐1 release in vitro and in vivo
Author(s) -
Perretti Mauro,
Becherucci Cristina,
Scapigliati Giuseppe,
Parente Luca
Publication year - 1989
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1989.tb12657.x
Subject(s) - in vivo , adrenalectomy , glucocorticoid , endocrinology , medicine , dexamethasone , prostaglandin e2 , lipopolysaccharide , in vitro , prostaglandin , interleukin , prostaglandin e , chemistry , cytokine , pharmacology , biology , biochemistry , microbiology and biotechnology
1 Peritoneal macrophages (Mø) collected from adrenalectomized (ADX) rats released more interleukin‐1 (IL‐1) activity and prostaglandin E 2 (PGE 2 ) than macrophages from sham‐operated (SHO) rats. 2 The increase in IL‐1 activity in the supernatants was confirmed by the increase of the cell‐associated 33 kD IL‐1α precursor in ADX macrophages stimulated by lipopolysaccharide (LPS). 3 After the injection of Complete Freund's Adjuvant (CFA) to induce adjuvant arthritis, 60% of the ADX rats died, while no deaths occurred in the SHO group. 4 The in vivo administration of dexamethasone inhibited both IL‐1 and PGE 2 release by macrophages as well as protecting ADX animals from CFA‐induced death. Indomethacin and BW 755C partially protected the animals from this lethal effect. 5 These results suggest that adrenalectomy induces an increased release of IL‐1 both in vitro and in vivo , and are consistent with a feedback mechanism between IL‐1 and glucocorticoid hormones.