Premium
Effect of reserpine on the subcellular distribution of 3 H‐α‐methylnoradrenaline in the mouse heart
Author(s) -
LUNDBORG P.
Publication year - 1969
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1969.tb09514.x
Subject(s) - reserpine , chemistry , endocrinology , medicine , alpha (finance) , amine gas treating , biology , organic chemistry , construct validity , nursing , patient satisfaction
1 . Mice were injected with reserpine (0.5 mg/kg or 2.5 mg/kg) at various intervals after an intravenous injection of 3 H‐α‐methylnoradrenaline ( 3 H‐α‐MeNa), 20 μg or 100 μg/kg. The effect of reserpine on the content of labelled amine in the subcellular fractions of the heart was studied. 2 . In most experiments reserpine caused a pronounced decrease in the 3 H‐α‐MeNA content of the particulate fraction (P). The decrease was most pronounced when the higher dose of reserpine and lower dose of α‐MeNA were used. In most experiments, the 3 H‐α‐MeNA content of the supernatant fractions was unchanged and, therefore, the P/(P + S) ratio was decreased. 3 . It is concluded that the effect of reserpine is a releasing effect on the particulate (granular) fraction, probably by blocking the re‐uptake of the amine leaked from the granules. No support was found for the hypothesis that reserpine acts by inhibition of release of amines from the nerve granules.