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Antiviral activity of aqueous and ethanol extracts and of an isolated polysaccharide from Agaricus brasiliensis against poliovirus type 1
Author(s) -
Faccin L.C.,
Benati F.,
Rincão V.P.,
Mantovani M.S.,
Soares S.A.,
Gonzaga M.L.,
Nozawa C.,
Carvalho Linhares R.E.
Publication year - 2007
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2007.02153.x
Subject(s) - agaricus , mushroom , poliovirus , polysaccharide , microbiology and biotechnology , ethanol , aqueous solution , chemistry , food science , biology , edible mushroom , virus , traditional medicine , biochemistry , virology , organic chemistry , medicine
Abstract Aims: Agaricus brasiliensis (previously named Agaricus blazei ss. Heinem), also known as the sun mushroom is native of Southeast Brazil, and is widely consumed, mainly in the form of tea, due to its nutritional and pharmacological properties. In this study, we tested aqueous (AqE) and ethanol (EtOHE) extracts and an isolated polysaccharide (PLS) from the fruiting body of A. brasiliensis, for antiviral activity against poliovirus type 1 in HEp‐2 cells. Methods and Results: The evaluation of the time of addition by plaque assay showed that when AqE, PLS and EtOHE were added, just after the virus inoculation (time 0 h), there was a concentration‐dependent reduction in the number of plaques up to 50%, 67% and 88%, respectively, with a selectivity index (SI) of 5·4, 9·9 and 12·3, respectively. Conclusions: The test substances showed antiviral activity and were more effective when added during the poliovirus infection. As they had little effect on reducing viral adsorption and did not show any virucidal effect, we suggest that they act at the initial stage of the replication of poliovirus. Significance and Impact of the Study: These results corroborate that basidiomycetes can be a rich source of potential antiviral compounds.