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Variation in Salmonella core lipopolysaccharide as detected by the monoclonal antibody M105
Author(s) -
Mansfield L.P.,
Billett E.,
Olsen E.,
Forsythe S.J.
Publication year - 1996
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1996.tb00041.x
Subject(s) - monoclonal antibody , salmonella , lipopolysaccharide , microbiology and biotechnology , variation (astronomy) , biology , bacteria , enterobacteriaceae , core (optical fiber) , antibody , virology , escherichia coli , immunology , genetics , physics , gene , astrophysics , optics
L.P. MANSFIELD, E. BILLETT, E. OLSEN AND S.J. FORSYTHE. 1996. The lipopolysaccharide antigenicity of 22 Salmonella strains (representing nine serogroups) and four non‐salmonellae Enterobacteriaceae to the Salmonella genus specific monoclonal antibody M105 was analysed. The monoclonal antibody M105 reacted with all 22 Salmonella strains. Probing SDS‐PAGE separated LPS molecules with MAb M105 revealed that the antibody reacted with the core region of all Salmonella serovars. However, no reaction was obtained to the long‐chain LPS of serovars O ( Salm. adelaide and Salm. ealing ), C 1 ( Salm. infantis, Salm. livingstone and Salm. virchow ) or Salm. arizonae . It is plausible that the presence of a second core antigenic type results in the lack of reaction between long‐chain LPS and the Salmonella genus specific monoclonal antibody M105.