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Presence of glycerol teichoic acid in the cell wall of Lactobacillus kefiranofaciens
Author(s) -
Mukai T.,
Onose Y.,
Toba T.,
Itoh T.
Publication year - 1992
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1992.tb00716.x
Subject(s) - teichoic acid , trichloroacetic acid , yield (engineering) , cell wall , chemistry , biochemistry , polysaccharide , chromatography , materials science , peptidoglycan , metallurgy
The accessory polymer of the capsular polysaccharide of a ‘kefiran’‐producing Lactobacillus kefiranofaciens was studied. The teichoic acid of L. kefiranofaciens K 1 was extracted from the cell with 5% trichloroacetic acid (w v) at 5°C for 24 h and was purified by ion‐exchange chromatography on DEAE‐Toyopearl 650 M with a linear gradient of (NH 4 ) 2 CO 3 ; the yield was only 2%. The teichoic acid has a molecular weight of 22000 and is composed of D‐glucose, 2‐acetamide‐2‐deoxy‐D‐glucose, glycerol and phosphorus in a molar ratio of 1.0 : 2.0 : 2.3 : 1.1. The low yield of teichoic acid suggested that kefiran is the main accessory polymer in the cell‐wall of L. kefiranofaciens.