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Extracellular production of a heat‐stable somatic antigen by Bacillus thuringiensis
Author(s) -
Mike A.,
Ohba M.,
Aizawa K.
Publication year - 1990
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1990.tb00173.x
Subject(s) - precipitin , antiserum , bacillus thuringiensis , biology , microbiology and biotechnology , serotype , strain (injury) , ouchterlony double immunodiffusion , agar , immunodiffusion , agar plate , nutrient agar , antigen , bacteria , immunology , genetics , anatomy
Extracellular production of a heat‐stable somatic antigen (HSSA) by Bacillus thuringiensis subsp. dendrolimus strain T84A1‐A [flagellar (H) serotype 4a: 4b] was serologically detected. In Ouchterlony tests, the HSSA antiserum gave single precipitin lines against both untreated and heat‐treated culture supernatants. These two precipitin lines fused completely. When colonies of strain T84A1‐A were grown on nutrient agar plates containing the homologous HSSA antiserum, precipitin halos were formed around the colonies. Of 27 type strains of B. thuringiensis subspecies tested, only the type strains of B. thuringiensis subsp. sotto (H serotype 4a: 4b) and B. thuringiensis subsp. israelensis (H serotype 14) formed [precipitin halos on nutrient agar plates containing antiserum against the HSSA of strain T84A1‐A.