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Isolation and cross‐amplification of microsatellites in pink abalone ( Haliotis corrugata )
Author(s) -
DÍAZVILORIA N.,
PÉREZENRÍQUEZ R.,
FIOREAMARAL G.,
BURTON R. S.,
CRUZ P.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.02054.x
Subject(s) - abalone , biology , microsatellite , haliotis discus , linkage disequilibrium , zoology , population , allele , fishery , genetics , gene , haplotype , demography , sociology
Abstract Ten novel microsatellite loci were isolated in pink abalone, Haliotis corrugata, using (GT) 15 and (CT) 15 enriched genomic libraries. Two previously reported Haliotis kamtschatkana microsatellites cross‐amplified in H. corrugata . A set of 12 polymorphic microsatellites were evaluated in a wild population sample ( N = 49). The number of alleles ranged from two to 55, and the observed and expected heterozygosities ranged from 0.104 to 0.939 and from 0.213 to 0.982, respectively. Significant deviations from Hardy–Weinberg equilibrium at three loci and no linkage disequilibrium were observed. Haliotis corrugata microsatellites cross‐amplified in other abalone species, two in H. fulgens , and seven in H. rufescens .