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Development of polymorphic markers from expressed sequence tags of Manihot esculenta Crantz
Author(s) -
TANGPHATSORNRUANG S.,
SRAPHET S.,
SINGH R.,
OKOGBENIN E.,
FREGENE M.,
TRIWITAYAKORN K.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.02047.x
Subject(s) - biology , manihot esculenta , locus (genetics) , allele , genetics , sequence (biology) , botany , microbiology and biotechnology , gene
In this study, 49 primers were designed from sequences containing di‐, tri‐, tetra‐, penta‐ and hexanucleotide motifs with a minimum of four repeats and presence of motif size polymorphisms (insertion/deletion) from cassava ( Manihot esculenta Crantz) expressed sequence tags deposited in public sequence database. Each locus was subsequently screened on 29 M. esculenta Crantz obtained from 15 different countries. Cross‐amplification was tested with M. esculenta Crantz (ssp. flabellifolia ) and four different Manihot species, M. chlorosticta , M. carthaginensis , M. filamentosa and M. tristis . Of these, nine loci showed polymorphic profiles within M. esculenta Crantz, which revealed two to four alleles per locus. The average unbiased and direct count heterozygosities were 0.4901 and 0.5674, respectively.