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Sixteen polymorphic microsatellites in bighead carp ( Aristichthys nobilis ) and cross‐amplification in silver carp ( Hypophthalmichthys molitrix )
Author(s) -
CHENG L.,
LIU L.,
YU X.,
TONG J.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.02037.x
Subject(s) - bighead carp , biology , hypophthalmichthys , silver carp , microsatellite , locus (genetics) , genetic diversity , loss of heterozygosity , zoology , population , genetics , allele , fishery , gene , demography , sociology , fish <actinopterygii>
A (GT) n enriched partial genomic library of bighead carp ( Aristichthys nobilis ) was constructed by employing the (fast isolation by AFLP of sequences containing repeats) FIASCO protocol. Sixteen loci exhibited polymorphism with two to seven alleles/locus (mean 3.263) in a test population and the observed heterozygosity ranging from 0.100 to 0.690 (mean 0.392). Eleven of the 16 bighead carp microsatellites were found to be also polymorphic in silver carp. These polymorphic loci should provide sufficient level of genetic diversity to evaluate population structure of bighead carp.