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PERMANENT GENETIC RESOURCES: Isolation and characterization of microsatellite loci in the North Island brown kiwi, Apteryx mantelli
Author(s) -
JENSEN THOMAS,
NUTT KAREN J.,
SEAL BRUCE S.,
FERNANDES LUIZA B.,
DURRANT BARBARA
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.01970.x
Subject(s) - biology , microsatellite , kiwi , locus (genetics) , genetic diversity , genetics , polymerase chain reaction , biological dispersal , zoology , allele , evolutionary biology , ecology , gene , population , demography , sociology
We report the isolation and characterization of eight polymorphic and five monomorphic microsatellites in North Island brown kiwi (NIBK, Apteryx mantelli ), using two polymerase chain reaction (PCR) techniques employing either short‐tandem repeat primers (STR method) or random PCR‐based isolation of microsatellite arrays (PIMA method). Microsatellite polymorphism was subsequently determined using 65 individuals. There were two to seven alleles for each polymorphic locus with heterozygozity ranging between 0.04 and 0.86. These primers will be used in future studies to determine the level of extra‐pair copulation, dispersal patterns, and genetic diversity within and between wild populations of NIBK.