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Novel nuclear intron‐spanning primers for Arecaceae evolutionary biology
Author(s) -
BACON CHRISTINE D.,
FELTUS F. ALEX,
PATERSON ANDREW H.,
BAILEY C. DONOVAN
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.01928.x
Subject(s) - arecaceae , biology , evolutionary biology , intron , genetics , palm , gene , physics , quantum mechanics
In this study, 96 nuclear ‘conserved intron‐scanning primers’ were screened across subfamilies the Arecaceae (palms) for potential use in research focused on palm evolutionary biology. Primers were evaluated based on their ability to amplify single polymerase chain reaction products in Arecaceae, the clarity of sequencing reads, and the interspecific variability observed. Ultimately, the results suggest that: (i) seven of the loci are likely to be suitable when comparing non‐Arecaceae outgroups and Arecaceae ingroups; (ii) seven loci may be of use when comparing subfamilies of Arecaceae; and (iii) four of the loci may be of use when comparing closely related genera.