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Isolation and identification of eight microsatellite loci in the Cherokee darter ( Etheostoma scotti ) and their variability in other members of the genera Etheostoma, Ammocrypta, and Percina
Author(s) -
GABEL JENNIFER M.,
DAKIN ELIZABETH E.,
FREEMAN BYRON J.,
PORTER BRADY A.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.01903.x
Subject(s) - etheostoma , biology , microsatellite , zoology , evolutionary biology , population , genetics , fish <actinopterygii> , gene , fishery , allele , demography , sociology
The Cherokee darter Etheostoma scotti is a federally threatened fish endemic to the Etowah River system of northwest Georgia. In order to analyse the population structure and genetic diversity of this fish, eight tetranucleotide microsatellite genetic markers were developed. The marker set was applied to 13 additional darter species to test cross‐species amplification and polymorphism. Successful amplification was obtained for all eight loci in each of the 13 other species of darters, with between seven and eight polymorphic loci per species.