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Detection of Several Novel γ‐Aminobutyric Acid‐Containing Compounds in Human CSF
Author(s) -
Miller Joanne M.,
Ferraro Thomas N.,
Hare Theodore A.
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb04558.x
Subject(s) - hydrolysis , aminobutyric acid , chemistry , chromatography , fraction (chemistry) , high performance liquid chromatography , acid hydrolysis , alkaline hydrolysis , biochemistry , receptor
γ‐Aminobutyric acid (GABA) concentrations in human CSF are known to increase significantly after hydrolysis; however, the source of this increase has been unknown. Using either ion‐exchange or reverse‐phase chromatography coupled with on‐line alkaline hydrolysis, we have shown 2‐pyrrolidinone, the lactam of GABA, to be present in insufficient quantity to account for this increase. Subsequent experiments involving fraction collection of column eluents followed by acid hydrolysis and rechromatography demonstrated the presence of several previously undetected GABA‐containing compounds.