Premium
Distribution of Activity Converting 4‐Aminobutyraldehyde to γ‐Aminobutyric Acid in Subcellular Fractions of Mouse Brain
Author(s) -
Asakura Tadashi,
Ikeda Yoshitaka,
Matsuda Makoto
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb09141.x
Subject(s) - mitochondrion , cytosol , biochemistry , sucrose gradient , choline acetyltransferase , glutamic acid , glutamate decarboxylase , synaptosome , distribution (mathematics) , acetylcholinesterase , chemistry , biology , enzyme , amino acid , acetylcholine , endocrinology , mathematical analysis , mathematics , membrane
Abstract: The subcellular distribution of activity of 4‐amino‐butyraldehyde dehydrogenase (ABAL‐DH) was studied in mouse brain. ABAL‐DH was localized mainly in the crude mitochondrial fraction; most of the activity in this fraction was found in the subfraction containing synaptosomes, and the remainder was in the mitochondrial fraction. After osmotic disruption of synaptosomes, most of the activity was located in the synaptic cytosol, and the remainder was in the synaptic mitochondria. Sucrose density subfractionation of synaptosomes revealed that γ‐aminobutyric acid, glutamic acid decarboxylase, and ABAL‐DH localized in a denser region of gradient fraction than the region containing acetylcholinesterase and choline acetyltransferase.