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Measurement of Free Intracellular Calcium in the Brain by 19 F‐Nuclear Magnetic Resonance Spectroscopy
Author(s) -
Bachelard H. S.,
BadarGoffer R. S.,
Brooks K. J.,
Dolin S. J.,
Morris P. G.
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb03102.x
Subject(s) - intracellular , calcium , nuclear magnetic resonance , chemistry , nuclear magnetic resonance spectroscopy , calcium in biology , biophysics , spectroscopy , depolarization , analytical chemistry (journal) , biochemistry , chromatography , biology , physics , organic chemistry , quantum mechanics
Abstract: We report the first measurement of the free intracellular calcium level in an actively metabolising intact cerebral tissue preparation. To this end, we applied the recently developed 19 F‐nuclear magnetic resonance calcium chelator, 5,5′‐F 2 ‐1,2‐bis( o ‐aminophenoxy)ethane‐ N,N,N′,N ′‐tetraacetic acid (5FBAPTA), in superfused cerebral cortical slices to give values for the intracellular Ca 2+ concentration of 350 and 480 n M , at external calcium concentrations of 1.2 and 2.4 m M , respectively. Under both conditions, the intracellular Ca 2+ concentration was increased by depolarisation using a high external K + concentration. Interleaved 31 P spectra showed that the presence of the 5FBAPTA had a deleterious effect on the metabolic state of the tissue with an external Ca 2+ concentration of 1.2 m M , but normal viability was maintained using 2.4 mM .