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Dibutyryl‐Cyclic GMP Stimulation of Ca 2+ ‐ATPase Activity in Rat Brain Synaptic Membranes
Author(s) -
Stauderman Kenneth A.,
Jones David J.,
Ross David H.
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb04089.x
Subject(s) - atpase , stimulation , synaptosome , lysis , cerebellum , medicine , chemistry , enzyme , endocrinology , membrane , biophysics , biochemistry , biology
The effects of dibutyryl cyclic AMP (dbcAMP) and dibutyryl cyclic GMP (db‐cGMP) were tested on Ca 2+ ‐ATPase, Mg 2+ ‐ATPase, and (Ca 2+ + Mg 2+ )‐ATPase activities in lysed synaptosomes prepared from whole rat brains (minus cerebellum). At concentrations from 0.1 to 2.0 m M , db‐cGMP produced a selective, concentration‐dependent increase in Ca 2+ ‐ATPase activity. Both db‐cGMP and db‐cAMP slightly reduced Mg 2+ ‐ATPase activity, whereas neither compound had concentration‐dependent effects on (Ca 2+ + Mg 2+ )‐ATPase activity. These findings suggest that the Mg 2+ ‐independent, Ca 2+ ‐ATPase activity in rat brain is regulated by a cyclic GMP‐dependent process. Further, the data provide evidence that the Ca 2+ ‐ATPase activity in lysed synaptosomal membranes represents an enzyme that is distinguishable from both the Mg 2+ ‐ and (Ca 2+ + Mg 2+ ‐ATPase.