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Irreversible Inhibition of Calcium Uptake in Synaptosomes by Quinacrine Mustard: Relationship to Labeling Sites of Quinacrine Mustard
Author(s) -
Ohta Akira,
Baba Akemichi,
Iwata Heitaroh
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb04041.x
Subject(s) - depolarization , calcium , chemistry , biochemistry , membrane , synaptosome , fluorescence , mustard plant , pharmacology , biophysics , biology , botany , organic chemistry , brassica , physics , quantum mechanics
The sites of interaction of quinacrine with synaptic membranes were labeled with quinacrine mustard. Quinacrine mustard had an inhibitory effect on depolarization‐induced calcium uptake by synaptosomes similar to that of quinacrine. The inhibition of depolarization induced calcium uptake by quinacrine was reduced by 70% after washing, whereas that by quinacrine mustard was not affected. Fluorescence electrophoretograms of the quinacrine mustard‐treated synaptic membranes showed that quinacrine mustard specifically labeled two proteins, with corresponding molecular weights of about 37,000 and 32,000.