Premium
Metabolic Processes in Alzheimer's Disease: Adenine Nucleotide Content and Production of 14 CO 2 from [U‐ 14 C]Glucose In Vitro in Human Neocortex
Author(s) -
Sims N. R.,
Bowen D. M.,
Neary D.,
Davison A. N.
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb00829.x
Subject(s) - neocortex , energy charge , in vitro , nucleotide , glycolysis , metabolism , adenine nucleotide , biochemistry , carbohydrate metabolism , human brain , chemistry , adenylate kinase , endocrinology , biology , enzyme , neuroscience , gene
Samples of neocortex removed at diagnostic craniotomy from patients with Alzheimer's disease and incubated in vitro showed an increased production of 14 CO 2 from [U‐ 14 C]glucose compared with neurosurgical controls. This was a feature of incubations in the presence of both 5 m M K + (142% control) and 31 mM K + (126%). Specific labelling of the amino acid pool was unaltered, suggesting that the apparent increase of CO 2 production was not merely a reflection of changes in dilution of the radiolabel from glucose. The content of adenine nucleotides was significantly less than control values in the tissue from patients with Alzheimer's disease after in vitro incubations but the adenylate energy charge was unchanged, indicating that normal energy metabolism was not grossly impaired in these preparations.