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Rapid Cellular Regulation of D‐Glucose Transport in Cultured Neural Cells
Author(s) -
Lange Klaus,
Keller Konrad,
Ludwig WolfDieter,
Monden Ingrid,
Reinsch Ingeborg,
Brandt Ursula
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb07992.x
Subject(s) - chemistry , microbiology and biotechnology , glucose transporter , neuroscience , biophysics , biology , endocrinology , insulin
Previous studies have revealed two different kinds of regulation of glucose utilization in cell lines derived from the nervous system (Keller et al., 1981). We found glucose metabolism of C‐6 glioma cells to be limited and regulated by membrane transport. In contrast, glucose utilization of C‐1300 neuroblastoma (N2A) cells was limited by the known regulatory enzymes of the Embden‐Meyerhof pathway. Under the given experimental conditions the “membrane‐limited” C‐6 glioma cells were characterized by periodically changing glucose transport rates and very low intracellular glucose concentrations, which remained constant in spite of widely differing transport rates. These findings suggest the close functional coupling between transport and phosphorylation required for the regulation of glucose transport by cellular metabolic needs.