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Uridine Metabolism in the Goldfish Retina During Optic Nerve Regeneration: Whole Retina Studies
Author(s) -
Dokas Linda A.,
Kohsaka Shinichi,
Burrell Harry R.,
Agranoff Bernard W.
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb01713.x
Subject(s) - uridine , retina , nucleoside , retinal , biochemistry , optic nerve , nucleotide , deoxyadenosine , biology , metabolism , chemistry , adenosine , rna , anatomy , neuroscience , gene
Accumulation of radioactivity from [ 3 H]uridine in incubations of whole goldfish retinas is increased in the ipsilateral retina during a period of regeneration that follows unilateral optic nerve crush. Brief incubations to investigate the nature of enhanced labeling of the acid‐soluble fraction showed a peak uptake 4 days following crush, with a gradual decrease to control levels by 21 days following crush. That nucleoside uptake may not mediate the effect is supported by the observation that the rate of uptake of 5′‐deoxyadenosine, a nonmetabolizable nucleoside analog, is the same in post‐crush (PC) and normal (N) retinal incubations. Following brief incubations of PC and N retinas with [ 3 H]uridine, there is enhanced labeling in PC retinas relative to N retinas of recovered UMP, UDP, UTP, and uridine nucleotide sugars, whereas recovery of labeled uridine itself is slightly decreased. The results suggest that the increased accumulation of radioactivity in PC retinas following incubation with uridine reflects an increase in the activities of retinal uridine kinase and uridine nucleotide kinases.