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KINETICS OF THE INCORPORATION OF SOME TRITIATED NUCLEIC ACID PRECURSORS AND [ 3 H]LYSINE INTO MOUSE BRAIN CELLS FOLLOWING INTRAPERITONEAL INJECTION
Author(s) -
Pakkenberg H.,
Fog R.
Publication year - 1973
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1973.tb07528.x
Subject(s) - uridine , guanosine , choroid plexus , neuropil , intraperitoneal injection , cytidine , labelling , microbiology and biotechnology , chemistry , thymidine , biochemistry , uracil , cytoplasm , biology , endocrinology , rna , central nervous system , dna , gene , enzyme
Abstract— Intraperitoneal injection into white mice of the same amount of radioactivity (0.5 mCi) of [ 3 H]uridine and [ 3 H]lysine demonstrated by autoradiography that there was a much greater labelling of nerve cells from lysine than from uridine. For uridine, the choroid plexus cell nuclei gave maximal labelling within 1 h, with a decrease after 6 h. The plexus nuclei of lysine‐injected animals gave almost the same amount of labelling during the experimental period of 48 h. In nerve cells, labelling from uridine increased in the nuclei up to 18 h after injection and there was an almost parallel increase in the labelling in the cytoplasm and neuropil. These results are compared with earlier reports on the results from intravenous injection of uridine. In lysine‐injected animals the nerve cell nuclei and cytoplasm showed a fairly constant amount of label over 48 h, but the neuropil counts increased steeply. The activity of the blood was determined by scintillation counting during the 48‐h period, and, as with uridine injection, was found to be almost constant over this period. A small series of animals was injected with 0.5 mCi of [ 3 H]uracil, [ 3 H]guanine, [ 3 H]guanosine or [ 3 H]cytidine for comparison. The autoradiograms from animals injected with these bases showed very slight labelling; that from guanosine was heavy in plexus nuclei, slight in nerve cells, and from cytidine it was heavy in plexus cells and moderate in nerve cells.