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Effect of medium properties and additives on antibody stability and accumulation in suspended plant cell cultures
Author(s) -
Tsoi Bonny M.Y.,
Doran Pauline M.
Publication year - 2002
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.2002.tb01186.x
Subject(s) - antibody , mannitol , plant cell , cell culture , biology , in vitro , chemistry , growth medium , food science , biochemistry , immunology , bacteria , genetics , gene
Factors affecting antibody accumulation and stability were investigated in transgenic plant cell cultures. Whereas IgG 1 antibody was stably maintained in media used for animal cell culture, there was a rapid loss over a period of 1–2 h of antibody added to sterile plant culture media. Antibody stability in Gamborg's B5 medium was improved in the absence of Mn. Tobacco suspensions producing IgG 1 antibody were used to test various medium‐based strategies for improving antibody accumulation in plant culture. Even though growth was suppressed, antibody levels in the biomass and medium were increased in media containing mannitol at osmolalities up to ≈450 mOsm·(kg of water) −1 . Adding gibberellic acid and haemin to the cultures was also beneficial, but the effects were not as great as those obtained under hyperosmolar conditions. Moderate increases in antibody accumulation were found by culturing plant cells in B5 medium without Mn.