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Antigenicity of deoxyadenosine 5‐monophosphate cross‐linked with polyamine
Author(s) -
Ahmad Aftab,
Ahmad Shama,
Ali Asif
Publication year - 1998
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1998.tb01372.x
Subject(s) - antigenicity , deoxyadenosine , polyamine , biochemistry , chemistry , adenosine , biology , genetics , antibody
Deoxyadenosine 5‐monophosphate was cross‐linked to spermine using glutaraldehyde as cross‐linking reagent. The complex thus obtained was characterized by UV absorption spectroscopy, size‐exclusion chromatography and CD. The complex had a molecular mass of around 10200 Da. Antibodies were raised against this complex in rabbits and the titre obtained was found to be >1:3200. The fine specificity of the raised antibodies was checked by competition ELISA. The complex also bound to naturally occurring anti‐DNA autoantibodies, suggesting a possible role of the complex in the pathogenesis of systemic lupus erythematosus.