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Immobilization of glucose oxidase on poly‐(L‐lysine)‐modified polycarbonate membrane
Author(s) -
Suye Shinichiro,
Kumon Yuji,
Ishigaki Akira
Publication year - 1998
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1998.tb00501.x
Subject(s) - glucose oxidase , glutaraldehyde , membrane , immobilized enzyme , polycarbonate , lysine , chemistry , enzyme , thermal stability , chromatography , biochemistry , polymer chemistry , organic chemistry , amino acid
The paper describes the immobilization of glucose oxidase on a polycarbonate membrane modified by a urethane coupling with poly‐(L‐lysine) and activated with glutaraldehyde. The enzymic properties of the immobilized enzyme were investigated and compared with the those of native glucose oxidase. The thermal stability and pH stability of the immobilized glucose oxidase were greater than those of the native enzyme. The membrane was applied as a glucose sensor.