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Stabilizing effect of water/alcohol solvents towards autoxidation of glutaraldehyde‐modified haemoglobin
Author(s) -
NedjarArroume N.,
Castellano A.,
Guillochon D.
Publication year - 1995
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1995.tb00331.x
Subject(s) - autoxidation , glutaraldehyde , alcohol , chemistry , organic chemistry
Glutaraldehyde‐cross‐linked haemoglobin is potentially very attractive as a useful oxygen carrier for biomedical and technological purposes. However, its oxygen‐binding ability is restricted by the autoxidation of the iron which is faster than for native haemoglobin. We have studied the stabilization effect of ethanol, butan‐1‐ol, ethylene glycol and glycerol on the autoxidation of glutaraldehyde‐cross‐linked oxyhaemoglobin. We have shown that, in the presence of water/alcohol mixtures, cross‐linked haemoglobin became more stable towards autoxidation than native oxyhaemoglobin in an aqueous environment. From a structural point of view, an e.p.r. study showed that alcohols and cross‐linking had opposite effects on the haem environment. In contrast with the cross‐linking, alcohols stabilize oxyhaemoglobin by decreasing the charge transfer from the iron to the oxygen, decreasing the O‐O length and increasing the total distance (F8 His)‐N‐F‐O2.