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One‐step isolation of a chitinase by affinity chromatography of the chitinolytic enzyme complex produced by Streptomyces kurssanovii
Author(s) -
AV Ilyina,
VP Varlamov,
VE Tikhonov,
IA Yamskov,
VA Davankov
Publication year - 1994
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1994.tb00294.x
Subject(s) - chitinase , streptomyces , affinity chromatography , enzyme , homogeneous , chitin , chromatography , streptomycetaceae , chemistry , actinomycetales , biochemistry , biology , bacteria , chitosan , physics , genetics , thermodynamics
One of the four chitinases with a molecular mass of 42 kDa existing in the chitinolytic enzyme complex produced by Streptomyces kurssanovii was separated in homogeneous form using one‐step affinity chromatography on cross‐linked and phosphorylated chitin‐type sorbents. Three other chitinases were not selectively bound by the sorbents and were not obtained in homogeneous form. The affinity sorbents were shown to be stable over the time of separation and could be used repeatedly.