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Lipid hydroperoxide‐resistance gene in Saccharomyces cerevisiae: utilization as a selectable marker gene for yeast transformation
Author(s) -
Inoue Y.,
Kobayashi S.,
Yoshikawa K.,
Tran LT,
Kimura A.
Publication year - 1993
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1111/j.1470-8744.1993.tb00246.x
Subject(s) - selectable marker , saccharomyces cerevisiae , plasmid , transformation (genetics) , auxotrophy , recombinant dna , biology , gene , marker gene , yeast , dna , genetic marker , genetics , microbiology and biotechnology , biochemistry , mutant
Two kinds of DNA fragments concerned with resistance against lipid hydroperoxide were cloned from the yeast Saccharomyces cerevisiae. When the recombinant plasmid carrying the DNA fragment was introduced into the cells of S. cerevisiae which do not have an appropriate auxotrophic marker, the transformants could be selected by their resistance to t‐butyl hydroperoxide. By Southern hybridization analysis, such transformants were found to contain the recombinant plasmid, suggesting that the DNA fragment could be used as a selection marker for the transformation of S. cerevisiae.