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PHYSIOLOGICAL INTERACTIONS DURING THE INITIAL STAGES OF EMBRYOGENESIS IN CULTURES OF DAUCUS CAROTA L.
Author(s) -
WARREN GRAHAM S.,
FOWLER MICHAEL W.
Publication year - 1981
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1981.tb03220.x
Subject(s) - daucus carota , auxin , subculture (biology) , somatic embryogenesis , embryo , biology , botany , morphogenesis , embryogenesis , microbiology and biotechnology , biochemistry , gene
SUMMARY Somatic globular embryos of carrot develop abnormally when transferred at low density to fresh, minus‐auxin medium, whereas normal development ensues after transfer to conditioned medium, or to fresh medium at high densities. It is suggested that a critical level of an ‘embryo factor’ is required to co‐ordinate cell division and morphogenesis. Embryo death occurs after the heart stage in minus‐auxin cultures of low embryogenic potential. This behaviour may result from the production of auxin as these cultures show some plus‐auxin characteristics. The decline in the capacity for normal embryogenesis which often accompanies repeated subculture is suggested to result, in part, from a combination of these factors.