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REGENERATION IN ROOT CUTTINGS OF TARAXACUM OFFICINALE I. THE NATURE AND LEVELS OF ENDOGENOUS HORMONES
Author(s) -
BOOTH A.,
SATCHUTHANANTHAVALE R.
Publication year - 1974
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1974.tb02122.x
Subject(s) - auxin , gibberellin , kinetin , cytokinin , taraxacum officinale , bioassay , botany , cutting , chemistry , biology , chromatography , biochemistry , tissue culture , dandelion , in vitro , medicine , alternative medicine , traditional chinese medicine , pathology , genetics , gene
SUMMARY The levels of auxins, cytokinins and gibberellins in extracts of fresh roots of Taraxacum were estimated by bioassay after chromatography. Auxin activity, largely confined to a single zone on chromatograms, had the R F and chromogenic properties of IAA and ranged from 45 to 85 μg kg ‐1 fresh weight of roots (in IAA equivalents). Similar auxin activity was found in root diffusates collected in agar gel. Most of the cytokinin activity was present in the zone R F 0.6‐0.8 on chromatograms developed in a n‐butanol: acetic acid: water solvent. The cytokinin was not characterized but its level in kinetin equivalents was in excess of 500 μg kg ‐1 fresh weight of roots. Gibberellin activity was of the order of 1 μg kg ‐1 in GA 3 equivalents.