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EFFECTS OF 2‐DEOXY‐ d ‐GLUCOSE ON THE GROWTH AND METABOLISM OF PROTOTHECA ZOPFII KRÜGER
Author(s) -
RANA M. S.,
CASSELTON P. J.
Publication year - 1974
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1974.tb01293.x
Subject(s) - pentose phosphate pathway , fructose , biochemistry , carbohydrate metabolism , glucose 6 phosphate dehydrogenase , glycogen , metabolism , dehydrogenase , biology , glucose uptake , hexokinase , carbohydrate , chemistry , glycolysis , enzyme , endocrinology , insulin
S ummary Some effects of 2‐deoxy‐D‐glucose (2‐DG) on the growth and metabolism of Prototheca zopfii Krüger are described. 2‐DG inhibits the rate of growth of the alga in medium containing glucose as carbon source and inhibition is more marked when fructose replaces glucose. Growth in medium containing acetate is not affected by the presence of 2‐DG. The addition of 2‐DG to carbohydrate starved cells, in addition to glucose, fructose or acetate, causes a marked increase in oxygen uptake; this is attributed to assimilation of 2‐DG. 2‐DG‐6‐phosphate inhibits the in vitro activity of glucose‐6‐phosphate dehydrogenase (E.G. 1.1.1.49) and experiments with radioactive glucose suggest that 2‐DG inhibits the oxidative pentose phosphate pathway of glucose metabolism in intact cells. Other effects of 2‐DG include inhibitions of glycogen‐UDP glucosyltransferase activity in vitro and of fermentation in vivo.