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Human Physiology
Author(s) -
M. Wittwer,
H. Hoppeler,
H. Howald,
M. Fluck,
R. Billeter
Publication year - 2002
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1111/j.1469-7793.2002.tb00417.x
Subject(s) - citation , computer science , information retrieval , world wide web
The expression of a set of 588 widely expressed genes coding for proteins involved in a wide variety of cellular functions was studied on biopsies from m. vastus lateralis of seven highly endurance trained males (professional cyclists) and seven untrained males. Their average ◊O2,max was 78 ml O2 min kg (S.D. = 8.8) and 35 ml O2 min _1 kg (S.D. = 6.6), respectively. Biopsies were obtained from m. vastus lateralis using the Bergström technique (Bergström, 1962) with permission from the Ethical Committee of the University of Bern. Total RNA (0.7–2 mg) was obtained from cryostat sections (10–25 mg) of the biopsies and used to probe Clontech Atlas Human 588 arrays with P labelling (Wittwer et al. 2002).