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Simultaneous Monitoring of Vascular Contractility, Intracellular Ph and Intracellular Calcium in Isolated Rat Mesenteric Arteries; Effects of Weak Bases
Author(s) -
Otter Donna,
Austin Clare
Publication year - 2000
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1111/j.1469-445x.2000.00353.x
Subject(s) - contractility , mesenteric arteries , intracellular , calcium , intracellular ph , washout , chemistry , medicine , endocrinology , biophysics , anatomy , biology , artery , biochemistry
We report the first simultaneous measurements of pH i , [Ca 2+ ] i and tension, upon alteration of pH i , in isolated rat mesenteric arteries loaded with both carboxy SNARF and indo‐1. In these vessels (pre‐contracted by 30 mM KCl) alterations of pH i , by addition and subsequent washout of weak bases, produced complicated effects on tone. Although the changes in contractility did not mirror the changes in pH i they were, at all times, accompanied by parallel changes in [Ca 2+ ] i .