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Stability of 0.5% cidofovir stored under various conditions for up to 6 months
Author(s) -
Stiles Jean,
Gwin Wilson,
Pogranichniy Roman
Publication year - 2010
Publication title -
veterinary ophthalmology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.594
H-Index - 50
eISSN - 1463-5224
pISSN - 1463-5216
DOI - 10.1111/j.1463-5224.2010.00784.x
Subject(s) - cidofovir , saline , vial , titer , chemistry , virus , titration , virology , chromatography , medicine , anesthesia , inorganic chemistry
Abstract Objective To evaluate the effect of time, temperature and storage vial material on the antiviral activity of 0.5% cidofovir solution. Procedures Commercial 7.5% cidofovir solution for injection was diluted with normal saline to a 0.5% concentration. Aliquots were stored in plastic and glass vials at 4, −20, and −80 °C for 30, 60, 120, and 180 days. Antiviral activity against feline herpesvirus was evaluated in a virus titration assay at time zero (baseline) and at each subsequent time point. Results Cidofovir caused a fourfold log reduction in virus titer at baseline and at each time point and for each storage condition ( P < 0.001). Conclusion 0.5% cidofovir demonstrated stable antiviral activity when stored for up to 6 months in glass or plastic, at 4, −20, and −80 °C.