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Complete chloroform dechlorination by organochlorine respiration and fermentation
Author(s) -
Lee Matthew,
Low Adrian,
Zemb Olivier,
Koenig Joanna,
Michaelsen Astrid,
Manefield Mike
Publication year - 2012
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/j.1462-2920.2011.02656.x
Subject(s) - acetogenesis , methanogenesis , chloroform , biology , fermentation , dehalococcoides , dichloromethane , environmental chemistry , carbon dioxide , microbial population biology , bacteria , food science , methane , biochemistry , chemistry , ecology , organic chemistry , genetics , vinyl chloride , solvent , copolymer , polymer
Summary Chloroform (CF, CHCl 3 ) is a recalcitrant and toxic environmental pollutant. In this communication we report for the first time a microbial community capable of complete CF dechlorination by metabolic processes. Cultures derived from subsurface soil (3.5 m) could sustain complete dechlorination of CF at levels of least 360 µM at a rate of 40 µM per day. Scrutiny of CF dechlorination revealed two metabolic processes at work. First, CF was respired to dichloromethane (DCM, CH 2 Cl 2 ), which was then fermented to acetate, hydrogen and carbon dioxide. Elevated hydrogen partial pressures were found to inhibit the fermentation process. Interspecies hydrogen transfer was observed in the form of methanogenesis and acetogenesis. This suggests that the dechlorination process required syntrophic partners to maintain low hydrogen partial pressures. 13 C‐labelled DCM was employed to help elucidate the chemistry of the process and identify bacterial community members involved. CF respiring cultures, where emulsified vegetable oil was supplied as the electron donor and DCM fermenting cultures, where DCM was supplied as the sole organic carbon source were studied separately. Pyrosequencing of these cultures revealed Dehalobacter lineages as a predominant community member in both. Subsequent growth experiments confirmed that the proliferation of Dehalobacter was linked directly to both the dehalorespiration and dehalofermentation processes.