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Genetic analyses and molecular characterization of the pathways involved in the conversion of 2‐phenylethylamine and 2‐phenylethanol into phenylacetic acid in Pseudomonas putida U
Author(s) -
Arias Sagrario,
Olivera Elías R.,
Arcos Mario,
Naharro Germán,
Luengo José M.
Publication year - 2008
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/j.1462-2920.2007.01464.x
Subject(s) - phenylacetaldehyde , phenylacetic acid , pseudomonas putida , biology , biochemistry , pyrroloquinoline quinone , aldehyde dehydrogenase , catabolism , stereochemistry , operon , dehydrogenase , escherichia coli , cofactor , enzyme , chemistry , gene
Summary In Pseudomonas putida U two different pathways (Pea, Ped) are required for the conversion of 2‐phenylethylamine and 2‐phenylethanol into phenylacetic acid. The 2‐phenylethylamine pathway (PeaABCDEFGHR) catalyses the transport of this amine, its deamination to phenylacetaldehyde by a quinohaemoprotein amine dehydrogenase and the oxidation of this compound through a reaction catalysed by a phenylacetaldehyde dehydrogenase. Another catabolic route (PedS 1 R 1 ABCS 2 R 2 DEFGHI) is needed for the uptake of 2‐phenylethanol and for its oxidation to phenylacetic acid via phenylacetaldehyde. This implies the participation of two different two‐component signal‐transducing systems, two quinoprotein alcohol dehydrogenases, a cytochrome c , a periplasmic binding protein, an aldehyde dehydrogenase, a pentapeptide repeat protein and an ABC efflux system. Additionally, two accessory sets of elements (PqqABCDEF and CcmABCDEFGHI) are necessary for the operation of the main pathways (Pea and Ped). PqqABCDEF is required for the biosynthesis of pyrroloquinoline quinone (PQQ), a prosthetic group of certain alcohol dehydrogenases that transfers electrons to an independent cytochrome c ; whereas CcmABCDEFGHI is required for cytochrome c maturation. Our data show that the degradation of phenylethylamine and phenylethanol in P. putida U is quite different from that reported in Escherichia coli , and they demonstrate that PeaABCDEFGHR and PedS 1 R 1 ABCS 2 R 2 DEFGHI are two upper routes belonging to the phenylacetyl‐CoA catabolon.