Premium
Identification of a cis ‐acting element required for dendritic targeting of activity‐regulated cytoskeleton‐associated protein mRNA
Author(s) -
Kobayashi Hiroaki,
Yamamoto Sachiya,
Maruo Tomohiko,
Murakami Fujio
Publication year - 2005
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.2005.04508.x
Subject(s) - arc (geometry) , messenger rna , untranslated region , microbiology and biotechnology , biology , translation (biology) , p bodies , in situ hybridization , genetics , gene , geometry , mathematics
The mRNA encoding activity‐regulated cytoskeleton‐associated protein (Arc) is known to be targeted to dendritic regions that have received strong synaptic inputs. However, the cis‐ acting elements in Arc mRNA that mediate dendritic targeting have not been identified. To identify the dendritic targeting element (DTE) in rat Arc mRNA, we expressed reporter mRNAs containing various regions of Arc in primary hippocampal neurones and analysed their subcellular distribution by in situ hybridization. Here, we report that the 3′‐untranslated region of rat Arc mRNA contains a 350‐nucleotide DTE with strong dendritic targeting activity and another 370‐nucleotide sequence with weaker dendritic targeting activity. The 350‐nucleotide DTE does not share any obvious sequence similarity with other known DTEs previously reported.