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Comparison of tissue microarray and full section in immunohistochemistry of gastrointestinal stromal tumors
Author(s) -
Kwon Mi Jung,
Nam Eun Sook,
Cho Seong Jin,
Park Hye Rim,
Shin Hyung Sik,
Park Jun Ho,
Park Chan Heun,
Lee Won Jae
Publication year - 2009
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.2009.02465.x
Subject(s) - gist , immunohistochemistry , cd34 , pathology , tissue microarray , stromal cell , concordance , core (optical fiber) , core biopsy , stromal tumor , medicine , biology , cancer , materials science , breast cancer , stem cell , composite material , genetics
The aim of the present study was to compare the efficiency of tissue microarray (TMA) results using immunohistochemistry markers applied to a variety of core sizes and full sections of gastrointestinal stromal tumors (GIST) using performance measures such as core loss rate and concordance rate. Six primary antibody markers (c‐Kit, CD34, smooth muscle actin (SMA), S‐100, p53, and Ki‐67) were used with the TMA technique to analyze 0.6 mm, 2 mm, and 3 mm punch cores of GIST samples from 67 patients. No statistical association was found between core size and loss rate ( P = 0.512). TMA results for the 0.6 mm, 2 mm, and 3 mm core showed that all core sizes could statistically significantly reflect full sections with regard to c‐Kit, SMA, and S‐100 antibodies, but that the 3 mm core section was the most representative except for CD34. With regard to p53 and Ki‐67 staining, the 0.6 mm core section was not representative, but the 2 mm and 3 mm core sections could statistically significantly represent full section results. Among them, the 3 mm core section was more accurate than the 2 mm core section. Use of a single 3 mm core size in TMA is suitable for evaluating large numbers of protein and nuclear stains with regard to immunohistochemistry for GIST.