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High reactivity of monoclonal antibody (TO73) with human malignant tumor cell line
Author(s) -
Kakihara Toshio,
Fukuda Takeaki,
Yamada Toshiyuki,
Tanaka Atsushi,
Uchiyama Makoto,
Naito Makoto,
Emura Iwao
Publication year - 1997
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.1997.tb04549.x
Subject(s) - monoclonal antibody , vincristine , cell culture , antigen , cancer research , antibody , leukemia , intracellular , cell , pathology , medicine , immunology , chemistry , biology , chemotherapy , cyclophosphamide , biochemistry , genetics
The biological characteristics d a new monoclonal antibody (TO73) reacting with a vincristine‐resistant human leukemic cell line (KY‐VCR) were, evaluated. Immunological and electron‐immunological studies showed that TO73 reacted with the surface glycoprotein of KY‐VCR. TO73 was found to have no effect on cell growth and intracellular uptake of vincristine. In human neoplastic cell ilnes TO73 was found to react with 11 of 27 (41%) cell lines. With regard to de novo primary tumor with one exception, TO73 did not react with any of the examined primary tumor cells. The patient with TO73‐positive leukemia died of induction failure due to drug resistance. Complete remission was achieved in the other leukemic patients. These results indicate that TO73 antigen may be associated with immortalization of tumor cells and poor prognosis in some cases.