Premium
A new monoclonal antibody (IE8) reactive with dendritically shaped cells in the human tonsil
Author(s) -
Nagasaki Makoto,
Harada Takayuki,
Morikawa Shigeru
Publication year - 1995
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/j.1440-1827.1995.tb03455.x
Subject(s) - mantle zone , follicular dendritic cells , germinal center , monoclonal antibody , tonsil , pathology , antigen , immunohistochemistry , chemistry , biology , microbiology and biotechnology , antibody , immunology , antigen presenting cell , b cell , t cell , medicine , immune system
A new monoclonal antibody (mAb), 1E8 (IgG1, k), was obtained from a hybrldoma prepared by fusion of mouse myeloma cells (NS‐1) with splenic cells of mice immunized with a human B blastic malignant lymphoma cell line, HPE‐Ret‐3 (Ret‐3). The mAb showed a reactivity unrestricted to a specific cell lineage on flow cytometrical analysis of the reacthttty with human lympho‐hematopoietic cell lines. In peripheral blood, 1E8 reacted with the cells of all lineage, that is, lymphocytes, monocytes, granulocytes and platelets, even though its intenslty was very low by immunohistochem‐Istry. Immunohistochemical examination of human tonsil with 1E8 showed a characteristic staining pattern. Positive cells scattered in follicular (mantle zone and germinal center), parafollicular (T‐dependent area), subepithelial and interstitlal connective tissue areas. These positive cells seemed to be categorized into dendritically shaped cells (DSC), including dendrltic cells (DC) and a subpopulation of macrophages in follicles, interdigitating cells (IDC) and irregularly shaped mononuclear cells. The localization of 1E8 antigen staining was similar to that of integrin CDi l c, although its distribution on hematopoietic cell lines did not coincide with that of 1E8 antigen. lmmunobiochemical studies showed that 1E8 bound two cell surface proteins with molecular size of 70000–90000 and 35000 Da each. Consequently, 1E8 antigen might be a novel marker of DSC.