Open AccessCytofluorometric Determination of Nuclear DNA in Heart Muscles of Patients with Muscular Dystrophy
Author(s) -
Kuwamura Yasuko,
Shono Masayuki,
Hizawa Kazuo
Publication year - 1989
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1989.tb02485.x
Subject(s) - muscular dystrophy , myotonic dystrophy , dystrophy , atrophy , wasting , muscle hypertrophy , cardiac muscle , pathology , medicine , muscle contracture , biology , nuclear dna , anatomy , mitochondrial dna , genetics , gene
The degree of DNA polyploidy of cardiac muscle cells was investigated by cytofluorometry in 4 cases of progressive muscular dystrophy (DMP) and 2 cases of myotonic dystrophy (DM), and the results were compared with those for non dystrophic hearts of normal or increased weight. The heart muscle cells from all patients with these forms of dystrophy showed marked nuclear DNA hyperploidy reaching 16c, irrespective of cardiac hypertrophy or atrophy. In the non dystrophic group, DNA hypertrophy corresponding to that of dystrophy was only detected in cases of marked cardiac hypertrophy exceeding a weight of 400 g. The wasting of the respiratory musculature, deformity of the thorax and cardiac muscular atrophy appeared to be the principal factors causing DNA polyploidy in patients with muscular dystrophy. Acta Pathol Jpn 39: 566 572,1989.