Open AccessPROLIFERATION OF ITO CELLS (FAT‐STORING CELLS) IN ACUTE CARBON TETRACHLORIDE LIVER INJURY: A Light and Electron Microscopic Autoradiographic Study
Author(s) -
Enzan Hideaki
Publication year - 1985
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1985.tb01429.x
Subject(s) - carbon tetrachloride , endoplasmic reticulum , golgi apparatus , hepatic stellate cell , pathology , necrosis , intraperitoneal injection , cytoplasm , thymidine , liver cytology , chemistry , hepatocyte , lobules of liver , liver injury , biology , biochemistry , endocrinology , dna , medicine , organic chemistry , liver metabolism , in vitro
The proliferative activity of Ito cells in acute liver injury induced by carbon tetrachloride (CCl 4 ) was studied by light and electron microscopic autoradio‐graphy. At 48 hours after a single intraperitoneal injection of CCl 4 , the livers of the mice given vitamin A per os for preceding 9 days and those of the mice without vitamin A‐pretreatment were removed. Small tissue blocks of each group were respectively incubated at 37°CC for 1 hour in culture medium containing 3 H‐thymidine. After CC1 4 , injection, perisinusoidal and sinusoidal cells adjacent to centrilobular necrotic liver cells increased in number and size. Some of them were labelled by 3 H‐thymidine. On the other hand, the perisinusoidal and sinusoidal cells in the peripheral zone in which liver cells are not markedly degenerated nor necrotic showed no noticeable increase in number. They contained very few or no silver grains after 3 H‐thymidine. In control mice the labelling of perisinusoidal cells was hardly observed. Electron microscopic autoradiography revealed that most of the labelled perisinusoidal cells in the centrilobular zone possess characteristics of Ito cells in their location and in the fine structures such as the presence of small fat droplets, well‐developed rough endoplasmic reticulum, and Golgi complex in the cytoplasm. These findings indicate that Ito cells incorporate 3 H‐thymidine in DNA synthesis after hepatocellular necrosis resulting in cell proliferation. ACTA PATHOL. JPN. 35: 1301–1308, 1985.