Premium
Posters
Author(s) -
Lam, SK,
Tu, S,
Yang, Y,
Zeng, H,
Zou, B,
Lin, MC,
Wang, J,
Wong, BCY,
Kung, H,
Jiang, SH,
He, H
Publication year - 2005
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.2005.04102.x
Subject(s) - citation , medicine , library science , information retrieval , world wide web , computer science
Background and Aims XIAP-associated factor 1 (XAF1) hasrecently been shown to negatively regulate the caspase-inhibitingactivity of XIAP (X-linked IAP), thus, XAF1 may play a pivotal rolein cancer cell apoptosis. The XAF1 mRNA is expressed at low orundetectable levels in various cancer cell lines, suggesting that thegene silencing in cancer cells is important for malignant cell survival.The aim of this study is to examine the role of promoter and exon 1hypermethylation in the transcriptional silencing of XAF1. Methods (1) We treated the gastric cancer cell lines AGS, MKN-45, BCG823 and colon cancer cell lines SW1116, HCT 15, colo 205with 5¢-AZA-2¢-deoxycytidine (5¢-AZA), then the mRNA levels weredetected by RT-PCR. (2) Genomic DNAs were isolated from thesecell lines and human normal samples, the methylation status wasdetermined by bisulfite DNA sequencing analysis for 8 CpG siteslocated around exon 1 area (nucleotides -40 to 194).Results The XAF1 mRNA were detected in all six cell lines. Therelative mRNA level ranked HCT 15 < AGS < SW1116,BCG 823